Composite

Part:BBa_K3149001:Design

Designed by: Triana Mayra S�nchez Huerta   Group: iGEM19_TecMonterrey_GDL   (2019-10-16)


TRZ1 receptor under the control of the pOmpC promoter with iLov fluoresent reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 690
    Illegal AgeI site found at 802
    Illegal AgeI site found at 1351
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As an alternative, we employed GelRed, an alternative marker for DNA electrophoresis. GelRed is marked as less toxic and more sensitive than ethidium bromide (EtBr), becoming a priority during these procedures.


Source

genomic e.coli

References